《植物生理学报》 2012, 48(12): 1151-1166

拟南芥ABC转运类蛋白家族的分子进化、表达模式和蛋白功能网络预测分析

朱璐, 许杰, 张大兵*

上海交通大学生命科学技术学院, 上海200240

通信作者：张大兵；E-mail: zhangdb@sjtu.edu.cn；Tel: 021-34205073

摘　要：

ABC转运蛋白又称腺苷三磷酸结合盒转运蛋白(ATP-binding cassette transporters), 该基因家族是目前已知最大、最古 老的蛋白家族之一, 在植物中ABC转运蛋白种类繁多、结构复杂、功能多样, 涉及植物一切的生命活动过程。本研究系统 介绍了拟南芥中131个ABC转运蛋白的亚家族分类、系统命名、蛋白大小以及蛋白亚细胞定位等基因信息, 在此基础上, 分析了ABC转运蛋白基因在染色体分布以及进化过程中发生的复制事件; 其次在47个组织器官或发育阶段中聚类分析了 ABC转运蛋白的表达模式和各个亚家族分布规律, 结果表明ABC转运蛋白基因的表达具有明显的组织特异性和时空特异 性, 说明在进化过程中该类蛋白功能也进一步发生分化; 我们以花药发育过程为例, 说明ABC转运蛋白在花药发育过程中 具有较高的协调性, 在时空和组织上表达受到严格的调控; 最后我们分析了ABC转运蛋白亚家族内部和各个亚家族之间可 能存在的蛋白相互作用关系, 推测ABC半分子转运蛋白形成同源或异源二聚体发挥功能的可能性, 进一步说明ABC转运蛋 白在蛋白互作水平上也存在功能多样性和严格的调控关系。

关键词：拟南芥; ABC转运蛋白; 分子进化; 表达分析; 蛋白相互作用

收稿：2012-08-18   修定：2012-11-13

资助：自然科学基金面上项目(31000593)。

Molecular Evolution, Expression and Functional Network Prediction Analysis of ABC Transporter Gene Family in Arabidopsis thaliana

ZHU Lu, XU Jie, ZHANG Da-Bing*

School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China

Corresponding author: ZHANG Da-Bing; E-mail: zhangdb@sjtu.edu.cn; Tel: 021-34205073

Abstract:

ABC transporters (ATP-binding Cassette transporters) family is one of the biggest and oldest protein families, they have a variety of complex structure and diverse functions, participating in the various processed of plant growth and development. In this study, bioinformatics analysis identified 131 ABC transporters genes in Arabidopsis thaliana, a comprehensive overview of this gene family was presented, including the subfamily classification, gene names, protein size and sub-cellular localization. The 131 ABC transporters genes were grouped into 8 subfamilies on basis of phylogenetic relationship. Chromosome localization and gene duplication analysis suggest that segmental and retro-transposition-like event may result in the ABC transporters gene expansion. By analyzing the developmental expression pattern of ABC transporters genes of all the subfamilies in 47 organs or development stages in Arabidopsis, most of the ABC transporters genes were shown to be expressed in obvious tissues, specific developmental time, suggesting that the functions of these proteins differentiated during the evolution. We used anther development as one example and expression analysis showed highly coordinated expression of the ABC transporters. Moreover we predicted the possible interactions between ABC transporter proteins in every sub-family or among different ones using bioinformatic tool and speculate the possible functional forms ABC half-transporters as homodimer or heterodimer. This work is helpful in understanding the functional role of ABC transporters in plants.

Key words: Arabidopsis thaliana; ABC transporter; molecular evolution; expression analysis; protein-protein interaction